Journal: The Journal of Biological Chemistry

Article Title: Genetic Screen Reveals Link between the Maternal Effect Sterile Gene mes-1 and Pseudomonas aeruginosa- induced Neurodegeneration in Caenorhabditis elegans *

doi: 10.1074/jbc.M115.674259

Figure Lengend Snippet: Neurodegeneration phenotypes induced by P. aeruginosa infection. A, summary of the morphological changes observed in animals infected with P. aeruginosa. B, images of ASE neurons of strain OH4168 and the ASER neuron of strain OH3192 exposed to control E. coli or P. aeruginosa. C, images of the AWC neuron of strain CX5974 exposed to control E. coli or P. aeruginosa. Different morphological changes in the neurons are indicated by white arrows. A–C, synchronized L4 nematodes were cultured on plates containing full lawns of P. aeruginosa for 30 h at 25 °C and then visualized using a Leica M165 FC stereomicroscope. Scale bars, 50 μm. D, chemotaxis of L4 nematodes exposed to E. coli or P. aeruginosa. Asterisks indicate statistical significance (**, p < 0.01). The data represent the results of three independent experiments. Bars, mean ± S.E. (error bars).

Article Snippet: The animals were mounted onto agar pads and visualized using a Zeiss Axioscope microscope or Leica M165 FC microscope.

Techniques: Infection, Cell Culture, Chemotaxis Assay

Journal: The Journal of Biological Chemistry

Article Title: Genetic Screen Reveals Link between the Maternal Effect Sterile Gene mes-1 and Pseudomonas aeruginosa- induced Neurodegeneration in Caenorhabditis elegans *

doi: 10.1074/jbc.M115.674259

Figure Lengend Snippet: Effect of osmotic stress on survival and neurodegeneration. A, survival curves of OH3192 young adults grown on standard 50 mm NaCl or increasing concentrations of NaCl. B, neurodegeneration rates in OH3192 young adults cultured on plates containing 50 mm NaCl or increasing concentrations of NaCl for 30 h at 25 °C followed by visualization using a Leica M165 FC stereomicroscope. Asterisks indicate significant differences (**, p < 0.01; NS, not significant). The data represent the results of three independent experiments (n = 60 ± 5 animals). Bars, mean ± S.E. (error bars).

Article Snippet: The animals were mounted onto agar pads and visualized using a Zeiss Axioscope microscope or Leica M165 FC microscope.

Techniques: Cell Culture

Journal: The Journal of Biological Chemistry

Article Title: Genetic Screen Reveals Link between the Maternal Effect Sterile Gene mes-1 and Pseudomonas aeruginosa- induced Neurodegeneration in Caenorhabditis elegans *

doi: 10.1074/jbc.M115.674259

Figure Lengend Snippet: ac319 sterile animals are resistant to P. aeruginosa infection and P. aeruginosa-induced neurodegeneration. A, survival curves of OH3192, ac319 fertile, and ac319 sterile young adults exposed to P. aeruginosa. p < 0.0001, OH3192 versus ac319 sterile animals. B, images of the ASER neuron of OH3192, ac319 fertile, and ac319 sterile young adults exposed to P. aeruginosa. Scale bar, 50 μm. C, neurodegeneration rates of OH3192, ac319 fertile, and ac319 sterile young animals exposed to P. aeruginosa. Synchronized young adult animals were cultured on full lawn plates of P. aeruginosa for 30 h at 25 °C. The neurodegeneration rate of OH3192, ac319 fertile, and ac319 sterile animals was scored using a Leica M165 FC stereomicroscope. Asterisks indicate significant differences (*, p < 0.05; **, p < 0.01). The graph represents the combined results of three independent experiments (n = 60 ± 5 animals). Bars, mean ± S.E. (error bars).

Article Snippet: The animals were mounted onto agar pads and visualized using a Zeiss Axioscope microscope or Leica M165 FC microscope.

Techniques: Infection, Cell Culture

Journal: Journal of Optometry

Article Title: Correlations between clinical measures and symptoms: Report 1: Stereoacuity with accommodative, vergence measures, and symptoms

doi: 10.1016/j.optom.2020.02.002

Figure Lengend Snippet: Randot Stereoacuity test booklet. The bottom last plate scores up to 12.5 s arc.

Article Snippet: Stereoacuity tests measure the smallest amount of recognizable retinal disparity in seconds of arc and this was assessed using the Randot stereo test (RST) (Vision Assessment Corporation, New York, USA) which measures global and contour (local) stereopsis., The RST measures fine stereopsis (perception obtained from small retinal disparities that can be fused for single vision) and has been reported to be the most sensitive of the clinical test for stereoacuity and the reports on presence or absence of monocular cue is inconsistent., , The test booklet comprise symbols (house, square, apple and big circle) (measures from 400 to 100 s arc and smaller circles on test page , ( ).

Techniques:

Journal: Journal of Optometry

Article Title: Correlations between clinical measures and symptoms: Report 1: Stereoacuity with accommodative, vergence measures, and symptoms

doi: 10.1016/j.optom.2020.02.002

Figure Lengend Snippet: Sensitivity and specificity of diagnosis of defects in accommodative and vergence measures with Randot stereoacuity test.

Article Snippet: Stereoacuity tests measure the smallest amount of recognizable retinal disparity in seconds of arc and this was assessed using the Randot stereo test (RST) (Vision Assessment Corporation, New York, USA) which measures global and contour (local) stereopsis., The RST measures fine stereopsis (perception obtained from small retinal disparities that can be fused for single vision) and has been reported to be the most sensitive of the clinical test for stereoacuity and the reports on presence or absence of monocular cue is inconsistent., , The test booklet comprise symbols (house, square, apple and big circle) (measures from 400 to 100 s arc and smaller circles on test page , ( ).

Techniques: Biomarker Discovery